1hjk

ALKALINE PHOSPHATASE MUTANT H331QALKALINE PHOSPHATASE MUTANT H331Q

Structural highlights

1hjk is a 2 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
NonStd Res:
Gene:	PHOA ("Bacillus coli" Migula 1895)
Activity:	Alkaline phosphatase, with EC number 3.1.3.1
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Using a mutant version of E. coli alkaline phosphatase, we succeeded in trapping and determining the structure of the phospho-enzyme intermediate. The X-ray structure also revealed the catalytic water molecule, bound to one of the active site zinc ions, positioned ideally for the apical attack necessary for the hydrolysis of the intermediate.

Trapping and visualization of a covalent enzyme-phosphate intermediate.,Murphy JE, Stec B, Ma L, Kantrowitz ER Nat Struct Biol. 1997 Aug;4(8):618-22. PMID:9253408^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Murphy JE, Stec B, Ma L, Kantrowitz ER. Trapping and visualization of a covalent enzyme-phosphate intermediate. Nat Struct Biol. 1997 Aug;4(8):618-22. PMID:9253408

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OCA

[1] Murphy JE, Stec B, Ma L, Kantrowitz ER. Trapping and visualization of a covalent enzyme-phosphate intermediate. Nat Struct Biol. 1997 Aug;4(8):618-22. PMID:9253408

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