5meb

Crystal structure of yeast Cdt1 C-terminal domainCrystal structure of yeast Cdt1 C-terminal domain

Structural highlights

5meb is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CDT1_YEAST] DNA replication licensing factor, required for pre-replication complex assembly. Faithful duplication of the genetic material requires 'once per cell cycle' DNA replication initiation and elongation. Central to this control is the tightly regulated formation of prereplicative complexes (preRCs) at future origins of DNA replication. Required for the recruitment of the MCM2-7 helicase complex to the replication origins.^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7]

References

↑ Jacobson MD, Munoz CX, Knox KS, Williams BE, Lu LL, Cross FR, Vallen EA. Mutations in SID2, a novel gene in Saccharomyces cerevisiae, cause synthetic lethality with sic1 deletion and may cause a defect during S phase. Genetics. 2001 Sep;159(1):17-33. PMID:11560884
↑ Devault A, Vallen EA, Yuan T, Green S, Bensimon A, Schwob E. Identification of Tah11/Sid2 as the ortholog of the replication licensing factor Cdt1 in Saccharomyces cerevisiae. Curr Biol. 2002 Apr 16;12(8):689-94. PMID:11967159
↑ Randell JC, Bowers JL, Rodriguez HK, Bell SP. Sequential ATP hydrolysis by Cdc6 and ORC directs loading of the Mcm2-7 helicase. Mol Cell. 2006 Jan 6;21(1):29-39. PMID:16387651 doi:http://dx.doi.org/10.1016/j.molcel.2005.11.023
↑ Kawasaki Y, Kim HD, Kojima A, Seki T, Sugino A. Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells. 2006 Jul;11(7):745-56. PMID:16824194 doi:http://dx.doi.org/10.1111/j.1365-2443.2006.00975.x
↑ Asano T, Makise M, Takehara M, Mizushima T. Interaction between ORC and Cdt1p of Saccharomyces cerevisiae. FEMS Yeast Res. 2007 Dec;7(8):1256-62. Epub 2007 Sep 6. PMID:17825064 doi:http://dx.doi.org/10.1111/j.1567-1364.2007.00299.x
↑ Chen S, de Vries MA, Bell SP. Orc6 is required for dynamic recruitment of Cdt1 during repeated Mcm2-7 loading. Genes Dev. 2007 Nov 15;21(22):2897-907. PMID:18006685 doi:http://dx.doi.org/10.1101/gad.1596807
↑ Remus D, Beuron F, Tolun G, Griffith JD, Morris EP, Diffley JF. Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing. Cell. 2009 Nov 13;139(4):719-30. doi: 10.1016/j.cell.2009.10.015. Epub 2009 Nov, 5. PMID:19896182 doi:http://dx.doi.org/10.1016/j.cell.2009.10.015

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OCA

[1] Jacobson MD, Munoz CX, Knox KS, Williams BE, Lu LL, Cross FR, Vallen EA. Mutations in SID2, a novel gene in Saccharomyces cerevisiae, cause synthetic lethality with sic1 deletion and may cause a defect during S phase. Genetics. 2001 Sep;159(1):17-33. PMID:11560884

[2] Devault A, Vallen EA, Yuan T, Green S, Bensimon A, Schwob E. Identification of Tah11/Sid2 as the ortholog of the replication licensing factor Cdt1 in Saccharomyces cerevisiae. Curr Biol. 2002 Apr 16;12(8):689-94. PMID:11967159

[3] Randell JC, Bowers JL, Rodriguez HK, Bell SP. Sequential ATP hydrolysis by Cdc6 and ORC directs loading of the Mcm2-7 helicase. Mol Cell. 2006 Jan 6;21(1):29-39. PMID:16387651 doi:http://dx.doi.org/10.1016/j.molcel.2005.11.023

[4] Kawasaki Y, Kim HD, Kojima A, Seki T, Sugino A. Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells. 2006 Jul;11(7):745-56. PMID:16824194 doi:http://dx.doi.org/10.1111/j.1365-2443.2006.00975.x

[5] Asano T, Makise M, Takehara M, Mizushima T. Interaction between ORC and Cdt1p of Saccharomyces cerevisiae. FEMS Yeast Res. 2007 Dec;7(8):1256-62. Epub 2007 Sep 6. PMID:17825064 doi:http://dx.doi.org/10.1111/j.1567-1364.2007.00299.x

[6] Chen S, de Vries MA, Bell SP. Orc6 is required for dynamic recruitment of Cdt1 during repeated Mcm2-7 loading. Genes Dev. 2007 Nov 15;21(22):2897-907. PMID:18006685 doi:http://dx.doi.org/10.1101/gad.1596807

[7] Remus D, Beuron F, Tolun G, Griffith JD, Morris EP, Diffley JF. Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing. Cell. 2009 Nov 13;139(4):719-30. doi: 10.1016/j.cell.2009.10.015. Epub 2009 Nov, 5. PMID:19896182 doi:http://dx.doi.org/10.1016/j.cell.2009.10.015

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