Protein kinase Spk1

Function Protein kinase Spk1 (Rad53) is a serine/threonine protein kinase which phosphorylates proteins. Rad53 controls S-phase checkpoint and G1 and G2 DNA damage checkpoints. Rad53 phosphorylates proteins at serine, threonine and tyrosine residues. Structural highlights Rad53 contains phosphothreonine recognition domains: FHA1 at the N-terminal (residues 1-164) which selects for Asp at position +3 relative to phosphothreonine and FHA2 at the C-terminal (residues 573-730) which selects for Ile at position +3 relative to phosphothreonine. Two SCD - SQ/TQ-rich cluster domains – are flanking the kinase domain. The yeast FHA1 domain interacts with peptide containing phosphothreonine.

spinqualitylabelsall models Structure of yeast Rad53 FHA1 domain (grey) complex with phosphothreonine peptide (green) (PDB code 2a0t). Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D structures of protein kinase Spk13D structures of protein kinase Spk1

Updated on 14-July-2016

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• Rad53 FHA1 domain
  • 1k3j – yRad53 FHA1 domain – yeast - NMR
    
  • 1g3g, 1j4o – yRad53 FHA1 domain (mutant) - NMR
    
  • 1j4p, 1j4q, 1k3n, 1k3q – yRad53 FHA1 domain + Rad9 peptide - NMR
    
  • 1g6g, 2a0t, 2jqi – yRad53 FHA1 domain + phosphothreonine peptide
    
• Rad53 FHA2 domain
  • 1qu5, 1dmz, 1fhq – yRad53 FHA2 domain – yeast - NMR
    
  • 1fhr, 1j4k, 1j4l, 1k2m, 1k2n – yRad53 FHA2 domain + Rad9 peptide - NMR
    
• Rad53 kinase and SCD2 domains
  • 4pdp – yRad53 kinase and SCD2 domain (mutant)
    
  • 4pds – yRad53 kinase and SCD2 domain (mutant) + AMPPNP