4hpn

Crystal structure of a proposed galactarolactone cycloisomerase from Agrobacterium Tumefaciens, target EFI-500704, with bound Ca, ordered loopsCrystal structure of a proposed galactarolactone cycloisomerase from Agrobacterium Tumefaciens, target EFI-500704, with bound Ca, ordered loops

Structural highlights

4hpn is a 1 chain structure with sequence from Agrobacterium fabrum str. c58. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Gene:	Atu3139 (Agrobacterium fabrum str. C58)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[A9CEQ8_AGRT5] Catalyzes the ring opening of D-galactaro-1,4-lactone to yield 5-keto-4-deoxy-D-glucarate (KDG) via a beta-elimination reaction. This is a step in the oxidative degradation pathway of D-galacturonate, which allows A.tumefaciens to utilize D-galacturonate as a sole carbon source. To a lesser extent, can also use D-glucaro-1,4-lactone as substrate to produce KDG, but cannot use D-galactaro-1,5-lactone, D-glucaro-6,3-lactone and linear D-glucarate.^[1] ^[2]

References

↑ Andberg M, Maaheimo H, Boer H, Penttila M, Koivula A, Richard P. Characterization of a novel Agrobacterium tumefaciens galactarolactone cycloisomerase enzyme for direct conversion of D-galactarolactone to 3-deoxy-2-keto-L-threo-hexarate. J Biol Chem. 2012 May 18;287(21):17662-71. doi: 10.1074/jbc.M111.335240. Epub, 2012 Apr 5. PMID:22493433 doi:http://dx.doi.org/10.1074/jbc.M111.335240
↑ Bouvier JT, Groninger-Poe FP, Vetting M, Almo SC, Gerlt JA. Galactaro delta-lactone isomerase: lactone isomerization by a member of the amidohydrolase superfamily. Biochemistry. 2014 Feb 4;53(4):614-6. doi: 10.1021/bi5000492. Epub 2014 Jan 24. PMID:24450804 doi:http://dx.doi.org/10.1021/bi5000492

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OCA

[1] Andberg M, Maaheimo H, Boer H, Penttila M, Koivula A, Richard P. Characterization of a novel Agrobacterium tumefaciens galactarolactone cycloisomerase enzyme for direct conversion of D-galactarolactone to 3-deoxy-2-keto-L-threo-hexarate. J Biol Chem. 2012 May 18;287(21):17662-71. doi: 10.1074/jbc.M111.335240. Epub, 2012 Apr 5. PMID:22493433 doi:http://dx.doi.org/10.1074/jbc.M111.335240

[2] Bouvier JT, Groninger-Poe FP, Vetting M, Almo SC, Gerlt JA. Galactaro delta-lactone isomerase: lactone isomerization by a member of the amidohydrolase superfamily. Biochemistry. 2014 Feb 4;53(4):614-6. doi: 10.1021/bi5000492. Epub 2014 Jan 24. PMID:24450804 doi:http://dx.doi.org/10.1021/bi5000492

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