ConSurf Quick Analysis Procedure

Here is a quick procedure for coloring a protein molecule by evolutionary conservation. This procedure does not necessarily give the highest quality result, but will give you a preliminary result that is often sufficient. If you wish to take it further, see Limiting ConSurf Analysis to Proteins of a Single Function.

1. Go to the ConSurf Server.
2. Check Amino Acids.
3. Check Yes there is a known protein structure.
4. Enter your PDB code and click the Next button.
5. Choose one chain from the pull-down Chain Identifier menu. (ConSurf can only analyze one chain at a time.)