1w6y
CRYSTAL STRUCTURE OF A MUTANT W92A IN KETOSTEROID ISOMERASE (KSI) FROM PSEUDOMONAS PUTIDA BIOTYPE B
|
OverviewOverview
A structural motif called the small exterior hydrophobic cluster (SEHC), has been proposed to explain the stabilizing effect mediated by, solvent-exposed hydrophobic residues; however, little is known about its, biological roles. Unusually, in Delta(5)-3-ketosteroid isomerase from, Pseudomonas putida biotype B (KSI-PI) Trp92 is exposed to solvent on the, protein surface, forming a SEHC with the side-chains of Leu125 and Val127., In order to identify the role of the SEHC in KSI-PI, mutants of those, amino acids associated with the SEHC were prepared. The W92A, L125A/V127A, and W92A/L125A/V127A mutations largely decreased the conformational, stability, while the L125F/V127F mutation slightly increased the, stability, indicating that hydrophobic packing by the SEHC is important in, ... [(full description)]
About this StructureAbout this Structure
1W6Y is a [Single protein] structure of sequence from [Pseudomonas putida] with EQU and BME as [ligands]. Active as [Steroid Delta-isomerase], with EC number [5.3.3.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Small exterior hydrophobic cluster contributes to conformational stability and steroid binding in ketosteroid isomerase from Pseudomonas putida biotype B., Yun YS, Nam GH, Kim YG, Oh BH, Choi KY, FEBS J. 2005 Apr;272(8):1999-2011. PMID:15819891
Page seeded by OCA on Tue Oct 30 16:27:01 2007