1gpx
C85S GAPDX, NMR, 20 STRUCTURES
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OverviewOverview
The Fe2S2 cluster of the ferredoxin putidaredoxin (Pdx) can be replaced by, a single gallium ion, giving rise to a colorless, diamagnetic protein in, which, apart from the metal binding site, the major structural features of, the native ferredoxin are conserved. The solution structure of the C85S, variant of gallium putidaredoxin (C85S GaPdx), in which a non-ligand, cysteine is replaced by a serine, has been determined via multidimensional, NMR methods using uniformly 15N, 13C labeled samples of C85S GaPdx., Stereospecific assignments of leucine and valine methyl resonances were, made using 13C, 1H HSQC spectra obtained with fractionally 13C-labeled, samples, and backbone dihedral angle restraints were obtained using a, combination of two-dimensional J-modulated 15N, 1H HSQC and, ... [(full description)]
About this StructureAbout this Structure
1GPX is a [Single protein] structure of sequence from [Pseudomonas putida] with GA as [ligand]. Structure known Active Site: GAL. Full crystallographic information is available from [OCA].
ReferenceReference
The solution structure of a gallium-substituted putidaredoxin mutant: GaPdx C85S., Pochapsky TC, Kuti M, Kazanis S, J Biomol NMR. 1998 Oct;12(3):407-15. PMID:9835048
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