1e67
ZN-AZURIN FROM PSEUDOMONAS AERUGINOSA
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OverviewOverview
Azurin*, a by-product of heterologous expression of the gene encoding the, blue copper protein azurin from Pseudomonas aeruginosa in Escherichia, coli, was characterized by chemical analysis and electrospray ionization, mass spectrometry, and its structure determined by X-ray crystallography., It was shown that azurin* is native azurin with its copper atom replaced, by zinc in the metal binding site. Zinc is probably incorporated in the, apo-protein after its expression and transport into the periplasm., Holo-azurin can be reconstituted from azurin* by prolonged exposure of the, protein to high copper ion concentrations or unfolding of the protein and, refolding in the presence of copper ions. An X-ray crystallographic, analysis of azurin* at 0.21-nm resolution revealed that the overall, ... [(full description)]
About this StructureAbout this Structure
1E67 is a [Single protein] structure of sequence from [Pseudomonas aeruginosa] with ZN and NO3 as [ligands]. Structure known Active Sites: AC1, AC2, AC3, AC4 and AC5. Full crystallographic information is available from [OCA].
ReferenceReference
Characterization and crystal structure of zinc azurin, a by-product of heterologous expression in Escherichia coli of Pseudomonas aeruginosa copper azurin., Nar H, Huber R, Messerschmidt A, Filippou AC, Barth M, Jaquinod M, van de Kamp M, Canters GW, Eur J Biochem. 1992 May 1;205(3):1123-9. PMID:1576995
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