3pu0

Crystal Structure of a vesicular stomatitis virus nucleocapsid-polyC complexCrystal Structure of a vesicular stomatitis virus nucleocapsid-polyC complex

Structural highlights 3pu0 is a 6 chain structure with sequence from Vesicular stomatitis indiana virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: Related: 3pto, 3ptx, 3pu1, 3pu4 Gene: N (Vesicular stomatitis Indiana virus) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The genomic RNA of negative strand RNA viruses, such as vesicular stomatitis virus (VSV), is completely enwrapped by the nucleocapsid protein (N) in every stage of virus infection. During viral transcription/replication, however, the genomic RNA in the nucleocapsid must be accessible by the virus-encoded RNA-dependent RNA polymerase in order to serve as the template for RNA synthesis. With the VSV nucleocapsid and a nucleocapsid-like particle (NLP) produced in E. coli, we have found that the RNA in the VSV nucleocapsid can be removed by RNase A, in contrast to what was previously reported. Removal of the RNA did not disrupt the assembly of the N protein, resulting in an empty capsid. Poly-ribonucleotides could be re-encapsidated into the empty NLP and the crystal structures were determined. The crystal structures revealed variable degrees of association of the N protein with a specific RNA sequence. Access of RNA encapsidated in the nucleocapsid of vesicular stomatitis virus.,Green TJ, Rowse M, Tsao J, Kang J, Ge P, Zhou ZH, Luo M J Virol. 2010 Dec 22. PMID:21177817 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Nucleoprotein

spinqualitylabelsall models 3pu0, resolution 3.09Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image