3bci
|
Crystal Structure of Staphylococcus aureus DsbA
OverviewOverview
Bacterial Dsb proteins catalyse the in vivo formation of disulfide bonds, a critical step in the stability and activity of many proteins. Most studies on Dsb proteins have focused on Gram-negative bacteria and thus the process of oxidative folding in Gram-positive bacteria is poorly understood. To help elucidate this process in Gram-positive bacteria, DsbA from Staphylococcus aureus (SaDsbA) has been focused on. Here, the expression, purification, crystallization and preliminary diffraction analysis of SaDsbA are reported. SaDsbA crystals diffract to a resolution limit of 2.1 A and belong to the hexagonal space group P6(5) or P6(1), with unit-cell parameters a = b = 72.1, c = 92.1 A and one molecule in the asymmetric unit (64% solvent content).
About this StructureAbout this Structure
3BCI is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
ReferenceReference
Expression and crystallization of DsbA from Staphylococcus aureus., Heras B, Kurz M, Jarrott R, Byriel KA, Jones A, Thony-Meyer L, Martin JL, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Nov 1;63(Pt, 11):953-6. Epub 2007 Oct 24. PMID:18007049
Page seeded by OCA on Thu Feb 21 19:04:59 2008