4cgt
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DELETION MUTANT DELTA(145-150), F151D OF CYCLODEXTRIN GLYCOSYLTRANSFERASE
OverviewOverview
Bacterial cyclodextrin glycosyltransferases use starch to produce cyclic, maltooligosaccharides (cyclodextrins) which are of interest in various, applications. The cyclization reaction gives rise to a spectrum of ring, sizes consisting of predominantly six to eight glucosyl units. Using the, enzyme from Bacillus circulans strain no. 8, binding studies have been, performed with several substrates and analogues. The observed binding, modes differ in detail, but agree in general with data on homologous, enzymes. Based on these binding studies, two mutations were designed that, changed the production spectrum from the predominant product, beta-cyclodextrin of the wild-type enzyme towards gamma-cyclodextrin, which is of practical interest because it is rare and can encapsulate, larger nonpolar compounds.
About this StructureAbout this Structure
4CGT is a Single protein structure of sequence from Bacillus circulans with CA as ligand. Active as Cyclomaltodextrin glucanotransferase, with EC number 2.4.1.19 Full crystallographic information is available from OCA.
ReferenceReference
Substrate binding to a cyclodextrin glycosyltransferase and mutations increasing the gamma-cyclodextrin production., Parsiegla G, Schmidt AK, Schulz GE, Eur J Biochem. 1998 Aug 1;255(3):710-7. PMID:9738912
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