2ju2
Minimized mean solution structure of the acyl carrier protein domain from module 2 of 6-deoxyerythronolide B synthase (DEBS)
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OverviewOverview
Polyketides are a medicinally important class of natural products. The, architecture of modular polyketide synthases (PKSs), composed of multiple, covalently linked domains grouped into modules, provides an attractive, framework for engineering novel polyketide-producing assemblies. However, impaired domain-domain interactions can compromise the efficiency of, engineered polyketide biosynthesis. To facilitate the study of these, domain-domain interactions, we have used nuclear magnetic resonance (NMR), spectroscopy to determine the first solution structure of an acyl carrier, protein (ACP) domain from a modular PKS, 6-deoxyerythronolide B synthase, (DEBS). The tertiary fold of this 10-kD domain is a three-helical bundle;, an additional short helix in the second loop also contributes to the core, helical packing. Superposition of residues 14-94 of the ensemble on the, mean structure yields an average atomic RMSD of 0.64 +/- 0.09 A for the, backbone atoms (1.21 +/- 0.13 A for all non-hydrogen atoms). The three, major helices superimpose with a backbone RMSD of 0.48 +/- 0.10 A (0.99, +/- 0.11 A for non-hydrogen atoms). Based on this solution structure, homology models were constructed for five other DEBS ACP domains., Comparison of their steric and electrostatic surfaces at the putative, interaction interface (centered on helix II) suggests a model for, protein-protein recognition of ACP domains, consistent with the previously, observed specificity. Site-directed mutagenesis experiments indicate that, two of the identified residues influence the specificity of ACP, recognition.
About this StructureAbout this Structure
2JU2 is a Single protein structure of sequence from Saccharopolyspora erythraea. Active as Erythronolide synthase, with EC number 2.3.1.94 Full crystallographic information is available from OCA.
ReferenceReference
Solution structure and proposed domain domain recognition interface of an acyl carrier protein domain from a modular polyketide synthase., Alekseyev VY, Liu CW, Cane DE, Puglisi JD, Khosla C, Protein Sci. 2007 Oct;16(10):2093-107. PMID:17893358
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