1un5
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ARH-II, AN ANGIOGENIN/RNASE A CHIMERA
OverviewOverview
Human angiogenin (Ang) is a potent inducer of blood vessel formation and, is a member of the pancreatic ribonuclease superfamily. Its enzymatic, activity is unusually weak and biased toward cleavage after cytidine, nucleotides. As part of an ongoing investigation into the structural basis, of Ang's characteristic activity, we have determined the crystal, structures of three Ang variants having novel activity. (i) The structure, of T44D-Ang indicates that Asp44 can participate directly in pyrimidine, binding and that the intrinsic hydrogen-bonding capability of this residue, largely governs the pyrimidine specificity of this variant. Unexpectedly, the mutation also causes the most extensive disruption of the C-terminus, seen in any Ang variant thus far. This allows the side chain of Arg101 ... [(full description)]
About this StructureAbout this Structure
1UN5 is a [Single protein] structure of sequence from [Homo sapiens] with CIT as [ligand]. Active as [Pancreatic ribonuclease], with EC number [3.1.27.5]. Structure known Active Site: CIT. Full crystallographic information is available from [OCA].
ReferenceReference
Crystallographic studies on structural features that determine the enzymatic specificity and potency of human angiogenin: Thr44, Thr80, and residues 38-41., Holloway DE, Chavali GB, Hares MC, Baker MD, Subbarao GV, Shapiro R, Acharya KR, Biochemistry. 2004 Feb 10;43(5):1230-41. PMID:14756559
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