2frp
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Bacteriophage HK97 Expansion Intermediate IV
OverviewOverview
Maturation of the bacteriophage HK97 capsid from a precursor (Prohead II), to the mature state (Head II) involves a 60 A radial expansion. The mature, particle is formed by 420 copies of the major capsid protein organized on, a T = 7 laevo lattice with each subunit covalently crosslinked to two, neighbors. Well-characterized pH 4 expansion intermediates make HK97, valuable for investigating quaternary structural dynamics. Here, we use, X-ray crystallography and cryo-EM to demonstrate that in the final, transition in maturation (requiring neutral pH), pentons in Expansion, Intermediate IV (EI-IV) reversibly sample 14 A translations and 6 degrees, rotations relative to a fixed hexon lattice. The limit of this trajectory, corresponds to the Head II conformation that is secured at this extent, only by the formation of the final class of covalent crosslinks. Mutants, that cannot crosslink or EI-IV particles that have been rendered incapable, of forming the final crosslink remain in the EI-IV state.
About this StructureAbout this Structure
2FRP is a Single protein structure of sequence from Enterobacteria phage hk620. Full crystallographic information is available from OCA.
ReferenceReference
Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM., Gan L, Speir JA, Conway JF, Lander G, Cheng N, Firek BA, Hendrix RW, Duda RL, Liljas L, Johnson JE, Structure. 2006 Nov;14(11):1655-65. PMID:17098191
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