2f2h
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Structure of the YicI thiosugar Michaelis complex
OverviewOverview
For the first time, the thioglycoligase strategy has been successfully applied to -glycosidases. The -thioglycoligases derived from the family 31 glycosidases, -xylosidase from E. coli (YicI) and -glucosidase from Sulfolobus solfataricus, catalyze thioglycoligase reactions using -glycosyl fluorides and deoxythioglycosides as donors and acceptors, respectively, in yields up to 86%. In addition, we describe the Michaelis complex of YicI using one of the thioglycosides as a nonhydrolyzable substrate analogue and discuss the structural insights this yields into the specificity and mechanism of family 31 -glycosidases and the molecular basis of an associated genetic disease.
About this StructureAbout this Structure
2F2H is a Single protein structure of sequence from Escherichia coli with SO4, MPO, XTG and GOL as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Expanding the thioglycoligase strategy to the synthesis of alpha-linked thioglycosides allows structural investigation of the parent enzyme/substrate complex., Kim YW, Lovering AL, Chen H, Kantner T, McIntosh LP, Strynadka NC, Withers SG, J Am Chem Soc. 2006 Feb 22;128(7):2202-3. PMID:16478160
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