1zxa
Solution Structure of the Coiled-Coil Domain of cGMP-dependent Protein Kinase Ia
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OverviewOverview
Coiled-coil motifs play essential roles in protein assembly and molecular, recognition, and are therefore the targets of many ongoing structural and, functional studies. However, owing to the dynamic nature of many of the, smaller coiled-coil domains, crystallization for X-ray studies is very, challenging. Determination of elongated structures using standard NMR, approaches is inefficient and usually yields low-resolution structures due, to accumulation of small errors over long distances. Here we describe a, solution NMR approach based on residual dipolar couplings (RDCs) for rapid, and accurate structure determination of coiled-coil dimers. Using this, approach, we were able to determine the high-resolution structure of the, coiled-coil domain of cGMP-dependent protein kinase Ialpha, a protein of, previously unknown structure that is critical for physiological relaxation, of vascular smooth muscle. This approach can be extended to solve, coiled-coil structures with higher order assemblies.
About this StructureAbout this Structure
1ZXA is a Single protein structure of sequence from Homo sapiens. Active as Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 Full crystallographic information is available from OCA.
ReferenceReference
Rapid and accurate structure determination of coiled-coil domains using NMR dipolar couplings: application to cGMP-dependent protein kinase Ialpha., Schnell JR, Zhou GP, Zweckstetter M, Rigby AC, Chou JJ, Protein Sci. 2005 Sep;14(9):2421-8. PMID:16131665
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