1xmt

File:1xmt.gif

spinqualitylabelsall models 1xmt, resolution 1.150Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

X-ray structure of gene product from arabidopsis thaliana at1g77540

OverviewOverview

We describe X-ray crystal and NMR solution structures of the protein coded, for by Arabidopsis thaliana gene At1g77540.1 (At1g77540). The crystal, structure was determined to 1.15 A with an R factor of 14.9% (Rfree =, 17.0%) by multiple-wavelength anomalous diffraction using sodium bromide, derivatized crystals. The ensemble of NMR conformers was determined with, protein samples labeled with 15N and 13C + 15N. The X-ray structure and, NMR ensemble were closely similar with rmsd 1.4 A for residues 8-93., At1g77540 was found to adopt a fold similar to that of GCN5-related, N-acetyltransferases. Enzymatic activity assays established that At1g77540, possesses weak acetyltransferase activity against histones H3 and H4., Chemical shift perturbations observed in 15N-HSQC spectra upon the, addition of CoA indicated that the cofactor binds and identified its, binding site. The molecular details of this interaction were further, elucidated by solving the X-ray structure of the At1g77540-CoA complex., This work establishes that the domain family COG2388 represents a novel, class of acetyltransferase and provides insight into possible mechanistic, roles of the conserved Cys76 and His41 residues of this family.

About this StructureAbout this Structure

1XMT is a Single protein structure of sequence from Arabidopsis thaliana with BR as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Structure of Arabidopsis thaliana At1g77540 protein, a minimal acetyltransferase from the COG2388 family., Tyler RC, Bitto E, Berndsen CE, Bingman CA, Singh S, Lee MS, Wesenberg GE, Denu JM, Phillips GN Jr, Markley JL, Biochemistry. 2006 Dec 5;45(48):14325-36. PMID:17128971

Page seeded by OCA on Wed Nov 21 06:11:37 2007