1wjf
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SOLUTION STRUCTURE OF H12C MUTANT OF THE N-TERMINAL ZN BINDING DOMAIN OF HIV-1 INTEGRASE COMPLEXED TO CADMIUM, NMR, 40 STRUCTURES
OverviewOverview
The solution structure of His12 --> Cys mutant of the N-terminal zinc, binding domain (residues 1-55; IN(1-55)) of HIV-1 integrase complexed to, cadmium has been solved by multidimensional heteronuclear NMR, spectroscopy. The overall structure is very similar to that of the, wild-type N-terminal domain complexed to zinc. In contrast to the, wild-type domain, however, which exists in two interconverting, conformational states arising from different modes of coordination of the, two histidine side chains to the metal, the cadmium complex of the His12, --> Cys mutant exists in only a single form at low pH. The conformation of, the polypeptide chain encompassing residues 10-18 is intermediate between, the two forms of the wild-type complex.
About this StructureAbout this Structure
1WJF is a Single protein structure of sequence from Human immunodeficiency virus 1 with CD as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Solution structure of the His12 --> Cys mutant of the N-terminal zinc binding domain of HIV-1 integrase complexed to cadmium., Cai M, Huang Y, Caffrey M, Zheng R, Craigie R, Clore GM, Gronenborn AM, Protein Sci. 1998 Dec;7(12):2669-74. PMID:9865962
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