1uan
Crystal structure of the conserved protein TT1542 from Thermus thermophilus HB8
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OverviewOverview
The TT1542 protein from Thermus thermophilus HB8 is annotated as a, conserved hypothetical protein, and belongs to the DUF158 family in the, Pfam database. A BLAST search revealed that homologs of TT1542 are present, in a wide range of organisms. The TT1542 homologs in eukaryotes, PIG-L in, mammals, and GPI12 in yeast and protozoa, have, N-acetylglucosaminylphosphatidylinositol (GlcNAc-PI) de-N-acetylase, activity. Although most of the homologs in prokaryotes are hypothetical, and have no known function, Rv1082 and Rv1170 from Mycobacterium, tuberculosis are enzymes involved in the mycothiol detoxification pathway., Here we report the crystal structure of the TT1542 protein at 2.0 A, resolution, which represents the first structure for this superfamily of, proteins. The structure of the TT1542 monomer consists of a twisted, beta-sheet composed of six parallel beta-strands and one antiparallel, beta-strand (with the strand order 3-2-1-4-5-7-6) sandwiched between six, alpha-helices. The N-terminal five beta-strands and four alpha-helices, form an incomplete Rossmann fold-like structure. The structure shares some, similarity to the sugar-processing enzymes with Rossmann fold-like, domains, especially those of the GPGTF (glycogen phosphorylase/glycosyl, transferase) superfamily, and also to the NAD(P)-binding Rossmann fold, domains. TT1542 is a homohexamer in the crystal and in solution, the six, monomers forming a cylindrical structure. Putative active sites are, suggested by the structure and conserved amino acid residues.
About this StructureAbout this Structure
1UAN is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the conserved protein TT1542 from Thermus thermophilus HB8., Handa N, Terada T, Kamewari Y, Hamana H, Tame JR, Park SY, Kinoshita K, Ota M, Nakamura H, Kuramitsu S, Shirouzu M, Yokoyama S, Protein Sci. 2003 Aug;12(8):1621-32. PMID:12876312
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