1q0e
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Atomic resolution (1.15 ) crystal structure of bovine copper, zinc superoxide dismutase
OverviewOverview
Copper zinc superoxide dismutase (CuZnSOD) forms a crucial component of, the cellular response to oxidative stress by catalyzing the dismutation of, the superoxide radical to hydrogen peroxide and water. Mutations in human, CuZnSOD are associated with the development of familial amyotrophic, lateral sclerosis (motor neuron disease). We have determined the structure, of fully reduced bovine CuZnSOD to 1.15 A, the only atomic resolution, structure for an intact CuZnSOD and one of only a small number for, metalloproteins. For the first time, both subunits have been captured with, the three coordinate Cu(I) ligation required by the generally accepted, catalytic mechanism, where dismutation of the superoxide radical occurs, via reduction of Cu. Furthermore, the improved resolution compared to, previous studies (to 1.65 A) has allowed a more detailed examination of, the metal center environment and its associated water network in the, active site channel, facilitating the analysis of potential proton, transfer routes.
About this StructureAbout this Structure
1Q0E is a Single protein structure of sequence from Bos taurus with CU, ZN and ACE as ligands. Active as Superoxide dismutase, with EC number 1.15.1.1 Full crystallographic information is available from OCA.
ReferenceReference
Structure of fully reduced bovine copper zinc superoxide dismutase at 1.15 A., Hough MA, Hasnain SS, Structure. 2003 Aug;11(8):937-46. PMID:12906825
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