1pvb

Revision as of 01:01, 21 November 2007 by OCA (talk | contribs) (New page: left|200px<br /><applet load="1pvb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pvb, resolution 1.75Å" /> '''X-RAY STRUCTURE OF A...)
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X-RAY STRUCTURE OF A NEW CRYSTAL FORM OF PIKE 4.10 PARVALBUMIN

File:1pvb.jpg


1pvb, resolution 1.75Å

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OverviewOverview

A new crystal form of pike (pI 4.10) parvalbumin has been crystallized in, presence of EDTA at pH 8.0. The crystals are orthorhombic, space group, P2(1)2(1)2(1), with a = 51.84, b = 49.95, c = 34.96 A. Diffractometer data, were collected to 1.75 A. The structure was solved by molecular, replacement and refined to R = 0.168 for 7774 observed reflections [I>/=, 2sigma(I)] in the range 8.0-1.75 A. In spite of the presence of EDTA, calcium ions are present in both primary binding sites. As compared to the, previously reported structures, the main differences concern the, conformation of the N-terminal residues and the packing in the unit cell.

About this StructureAbout this Structure

1PVB is a Single protein structure of sequence from Esox lucius with CA, NH4 and ACE as ligands. Full crystallographic information is available from OCA.

ReferenceReference

X-ray structure of a new crystal form of pike 4.10 beta parvalbumin., Declercq JP, Tinant B, Parello J, Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):165-9. PMID:15299738

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