1noc
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MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DOMAIN (DELTA 114) COMPLEXED WITH TYPE I E. COLI CHLORAMPHENICOL ACETYL TRANSFERASE AND IMIDAZOLE
OverviewOverview
The nitric oxide synthase oxygenase domain (NOSox) oxidizes arginine to, synthesize the cellular signal and defensive cytotoxin nitric oxide (NO)., Crystal structures determined for cytokine-inducible NOSox reveal an, unusual fold and heme environment for stabilization of activated oxygen, intermediates key for catalysis. A winged beta sheet engenders a curved, alpha-beta domain resembling a baseball catcher's mitt with heme clasped, in the palm. The location of exposed hydrophobic residues and the results, of mutational analysis place the dimer interface adjacent to the, heme-binding pocket. Juxtaposed hydrophobic O2- and polar, L-arginine-binding sites occupied by imidazole and aminoguanidine, respectively, provide a template for designing dual-function inhibitors, and imply substrate-assisted catalysis.
About this StructureAbout this Structure
1NOC is a Protein complex structure of sequences from Escherichia coli and Mus musculus with HEM and IMD as ligands. Active as Nitric-oxide synthase, with EC number 1.14.13.39 Full crystallographic information is available from OCA.
ReferenceReference
The structure of nitric oxide synthase oxygenase domain and inhibitor complexes., Crane BR, Arvai AS, Gachhui R, Wu C, Ghosh DK, Getzoff ED, Stuehr DJ, Tainer JA, Science. 1997 Oct 17;278(5337):425-31. PMID:9334294
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