1mze
Human Factor Inhibiting HIF (FIH1)
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OverviewOverview
Precise regulation of the evolutionarily conserved hypoxia-inducible, transcription factor (HIF) ensures proper adaptation to variations in, oxygen availability throughout development and into adulthood., Oxygen-dependent regulation of HIF stability and activity are mediated by, hydroxylation of conserved proline and asparagine residues, respectively., Because the relevant prolyl and asparginyl hydroxylases use O(2) to effect, these posttranslational modifications, these enzymes are implicated as, direct oxygen sensors in the mammalian hypoxic response pathway. Here we, present the structure of factor-inhibiting HIF-1 (FIH-1), the pertinent, asparaginyl hydroxylase involved in hypoxic signaling. Hydroxylation of, the C-terminal transactivation domain (CTAD) of HIF by FIH-1 prevents CTAD, association with transcriptional coactivators under normoxic conditions., Consistent with other structurally known hydroxylases, FIH-1 is comprised, of a beta-strand jellyroll core with both Fe(II) and the cosubstrate, 2-oxoglutarate bound in the active site. Details of the molecular contacts, at the active site of FIH-1 have been elucidated and provide a platform, for future drug design. Furthermore, the structure reveals the presence of, a FIH-1 homodimer that forms in solution and is essential for FIH, activity.
About this StructureAbout this Structure
1MZE is a Single protein structure of sequence from Homo sapiens with TAR and FE2 as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Structure of factor-inhibiting hypoxia-inducible factor 1: An asparaginyl hydroxylase involved in the hypoxic response pathway., Dann CE 3rd, Bruick RK, Deisenhofer J, Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15351-6. Epub 2002 Nov 13. PMID:12432100
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