1kks
Structure of the histone mRNA hairpin required for cell cycle regulation of histone gene expression
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OverviewOverview
Expression of replication-dependent histone genes requires a conserved, hairpin RNA element in the 3' untranslated regions of poly(A)-less histone, mRNAs. The 3' hairpin element is recognized by the hairpin-binding protein, or stem-loop-binding protein (HBP/SLBP). This protein-RNA interaction is, important for the endonucleolytic cleavage generating the mature mRNA 3', end. The 3' hairpin and presumably HBP/SLBP are also required for, nucleocytoplasmic transport, translation, and stability of histone mRNAs., RNA 3' processing and mRNA stability are both regulated during the cell, cycle. Here, we have determined the three-dimensional structure of a, 24-mer RNA comprising a mammalian histone RNA hairpin using heteronuclear, multidimensional NMR spectroscopy. The hairpin adopts a novel UUUC, tetraloop conformation that is stabilized by base stacking involving the, first and third loop uridines and a closing U-A base pair, and by hydrogen, bonding between the first and third uridines in the tetraloop. The HBP, interaction of hairpin RNA variants was analyzed in band shift, experiments. Particularly important interactions for HBP recognition are, mediated by the closing U-A base pair and the first and third loop, uridines, whose Watson-Crick functional groups are exposed towards the, major groove of the RNA hairpin. The results obtained provide novel, structural insight into the interaction of the histone 3' hairpin with, HBP, and thus the regulation of histone mRNA metabolism.
About this StructureAbout this Structure
1KKS is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the histone mRNA hairpin required for cell cycle regulation of histone gene expression., Zanier K, Luyten I, Crombie C, Muller B, Schumperli D, Linge JP, Nilges M, Sattler M, RNA. 2002 Jan;8(1):29-46. PMID:11871659
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