1ire
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Crystal Structure of Co-type nitrile hydratase from Pseudonocardia thermophila
OverviewOverview
The crystal structure of cobalt-containing nitrile hydratase from, Pseudonocardia thermophila JCM 3095 at 1.8 A resolution revealed the, structure of the noncorrin cobalt at the catalytic center. Two cysteine, residues (alphaCys(111) and alphaCys(113)) coordinated to the cobalt were, posttranslationally modified to cysteine-sulfinic acid and to, cysteine-sulfenic acid, respectively, like in iron-containing nitrile, hydratase. A tryptophan residue (betaTrp(72)), which may be involved in, substrate binding, replaced the tyrosine residue of iron-containing, nitrile hydratase. The difference seems to be responsible for the, preference for aromatic nitriles rather than aliphatic ones of, cobalt-containing nitrile hydratase.
About this StructureAbout this Structure
1IRE is a Protein complex structure of sequences from Pseudonocardia thermophila with CO as ligand. Active as Nitrile hydratase, with EC number 4.2.1.84 Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of cobalt-containing nitrile hydratase., Miyanaga A, Fushinobu S, Ito K, Wakagi T, Biochem Biophys Res Commun. 2001 Nov 16;288(5):1169-74. PMID:11700034
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