1foy
THE RNA BINDING DOMAIN OF RIBOSOMAL PROTEIN L11: THREE-DIMENSIONAL STRUCTURE OF THE RNA-BOUND FORM OF THE PROTEIN, NMR, MINIMIZED AVERAGE STRUCTURE
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OverviewOverview
The three-dimensional solution structure has been determined by NMR, spectroscopy of the 75 residue C-terminal domain of ribosomal protein L11, (L11-C76) in its RNA-bound state. L11-C76 recognizes and binds tightly to, a highly conserved 58 nucleotide domain of 23 S ribosomal RNA, whose, secondary structure consists of three helical stems and a central junction, loop. The NMR data reveal that the conserved structural core of the, protein, which consists of a bundle of three alpha-helices and a, two-stranded parallel beta-sheet four residues in length, is nearly the, same as the solution structure determined for the non-liganded form of the, protein. There are however, substantial chemical shift perturbations which, accompany RNA binding, the largest of which map onto an extended loop, which bridges the C-terminal end of alpha-helix 1 and the first strand of, parallel beta-sheet. Substantial shift perturbations are also observed in, the N-terminal end of alpha-helix 1, the intervening loop that bridges, helices 2 and 3, and alpha-helix 3. The four contact regions identified by, the shift perturbation data also displayed protein-RNA NOEs, as identified, by isotope-filtered three-dimensional NOE spectroscopy. The shift, perturbation and NOE data not only implicate helix 3 as playing an, important role in RNA binding, but also indicate that regions flanking, helix 3 are involved as well. Loop 1 is of particular interest as it was, found to be flexible and disordered for L11-C76 free in solution, but not, in the RNA-bound form of the protein, where it appears rigid and adopts a, specific conformation as a result of its direct contact to RNA.
About this StructureAbout this Structure
1FOY is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.
ReferenceReference
The RNA binding domain of ribosomal protein L11: three-dimensional structure of the RNA-bound form of the protein and its interaction with 23 S rRNA., Hinck AP, Markus MA, Huang S, Grzesiek S, Kustonovich I, Draper DE, Torchia DA, J Mol Biol. 1997 Nov 21;274(1):101-13. PMID:9398519
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