1fnh
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CRYSTAL STRUCTURE OF HEPARIN AND INTEGRIN BINDING SEGMENT OF HUMAN FIBRONECTIN
OverviewOverview
The crystal structure of human fibronectin (FN) type III repeats 12-14, reveals the primary heparin-binding site, a clump of positively charged, residues in FN13, and a putative minor site approximately 60 A away in, FN14. The IDAPS motif implicated in integrin alpha4beta1 binding is at the, FN13-14 junction, rendering the critical Asp184 inaccessible to integrin., Asp184 clamps the BC loop of FN14, whose sequence (PRARI) is reminiscent, of the synergy sequence (PHSRN) of FN9. Mutagenesis studies prompted by, this observation reveal that both arginines of the PRARI sequence are, important for alpha4beta1 binding to FN12-14. The PRARI motif may, represent a new class of integrin-binding sites. The spatial organization, of the binding sites suggests that heparin and integrin may bind in, concert.
DiseaseDisease
Known diseases associated with this structure: Ehlers-Danlos syndrome, type X, 225310 (1) OMIM:[135600]
About this StructureAbout this Structure
1FNH is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of a heparin- and integrin-binding segment of human fibronectin., Sharma A, Askari JA, Humphries MJ, Jones EY, Stuart DI, EMBO J. 1999 Mar 15;18(6):1468-79. PMID:10075919
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