1aoo

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Revision as of 11:53, 20 November 2007 by OCA (talk | contribs) (New page: left|200px<br /><applet load="1aoo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1aoo" /> '''AG-SUBSTITUTED METALLOTHIONEIN FROM SACCHARO...)
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1aoo

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AG-SUBSTITUTED METALLOTHIONEIN FROM SACCHAROMYCES CEREVISIAE, NMR, MINIMIZED AVERAGE STRUCTURE

OverviewOverview

3D solution structural calculations for yeast silver(I)-substituted, metallothionein (MT) and native copper(I) MT were completed using, experimentally determined NOE and dihedral angle constraints, in, conjunction with experimentally derived metal-to-Cys connectivities for, AgMT which were assumed identical for CuMT. For the first 40 residues in, both structures, the polypeptide backbone wraps around the metal cluster, in two large parallel loops separated by a deep cleft containing the metal, cluster. Minor differences between the two structures include differences, in hydrogen bonds and the orientation of the N-terminus with the overall, protein volume conserved to within 6.5%.

About this StructureAbout this Structure

1AOO is a Single protein structure of sequence from Saccharomyces cerevisiae with AG as ligand. Full crystallographic information is available from OCA.

ReferenceReference

3D solution structure of copper and silver-substituted yeast metallothioneins., Peterson CW, Narula SS, Armitage IM, FEBS Lett. 1996 Jan 22;379(1):85-93. PMID:8566237

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