2hkq
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Crystal structure of the C-terminal domain of human EB1 in complex with the CAP-Gly domain of human Dynactin-1 (p150-Glued)
OverviewOverview
Dynamic microtubule plus-end tracking protein (+TIP) networks are, implicated in all functions of microtubules, but the molecular, determinants of their interactions are largely unknown. Here, we have, explored key binding modes of +TIPs by analyzing the interactions between, selected CAP-Gly, EB-like, and carboxy-terminal EEY/F-COO(-) sequence, motifs. X-ray crystallography and biophysical binding studies demonstrate, that the beta2-beta3 loop of CAP-Gly domains determines EB-like motif, binding specificity. They further show how CAP-Gly domains serve as, recognition domains for EEY/F-COO(-) motifs, which represent, characteristic and functionally important sequence elements in EB, CLIP-170, and alpha-tubulin. Our findings provide a molecular basis for, understanding the modular interaction modes between alpha-tubulin, CLIPs, EB proteins, and the dynactin-dynein motor complex and suggest that, multiple low-affinity binding sites in different combinations control, dynamic +TIP networks at microtubule ends. They further offer insights, into the structural consequences of genetic CAP-Gly domain defects found, in severe human disorders.
DiseaseDisease
Known diseases associated with this structure: Amyotrophic lateral sclerosis, susceptibility to OMIM:[601143], Neuropathy, distal hereditary motor, type VIIB OMIM:[601143]
About this StructureAbout this Structure
2HKQ is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Key interaction modes of dynamic +TIP networks., Honnappa S, Okhrimenko O, Jaussi R, Jawhari H, Jelesarov I, Winkler FK, Steinmetz MO, Mol Cell. 2006 Sep 1;23(5):663-71. PMID:16949363
Page seeded by OCA on Mon Nov 12 22:33:32 2007