2bya
IS RADIATION DAMAGE DEPENDENT ON THE DOSE-RATE USED DURING MACROMOLECULAR CRYSTALLOGRAPHY DATA COLLECTION
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OverviewOverview
This paper focuses on the radiation-damage effects when applying the same, total X-ray dose to protein crystals at different dose rates. These, experiments have been performed on both a selenomethionated protein and on, bovine trypsin using dose rates that span nearly two orders of magnitude., The results show no clear dose-rate effect on the global indicators of, radiation damage, but a small measurable dose-rate effect could be found, when studying specific radiation damage. It is hypothesized that this, observed dose-rate effect relates to differences in the steady-state, free-radical concentration.
About this StructureAbout this Structure
2BYA is a [Single protein] structure of sequence from [Bos taurus] with SO4, CA, BAM and GOL as [ligands]. Active as [[1]], with EC number [3.4.21.4]. Full crystallographic information is available from [OCA].
ReferenceReference
Is radiation damage dependent on the dose rate used during macromolecular crystallography data collection?, Leiros HK, Timmins J, Ravelli RB, McSweeney SM, Acta Crystallogr D Biol Crystallogr. 2006 Feb;62(Pt 2):125-32. Epub 2006, Jan 18. PMID:16421442
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