2j4d
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CRYPTOCHROME 3 FROM ARABIDOPSIS THALIANA
OverviewOverview
Cryptochromes are almost ubiquitous blue-light receptors and act in, several species as central components of the circadian clock. Despite, being evolutionary and structurally related with DNA photolyases, a class, of light-driven DNA-repair enzymes, and having similar cofactor, compositions, cryptochromes lack DNA-repair activity. Cryptochrome 3 from, the plant Arabidopsis thaliana belongs to the DASH-type subfamily. Its, crystal structure determined at 1.9 Angstroms resolution shows, cryptochrome 3 in a dimeric state with the antenna cofactor, 5,10-methenyltetrahydrofolate (MTHF) bound in a distance of 15.2 Angstroms, to the U-shaped FAD chromophore. Spectroscopic studies on a mutant where a, residue crucial for MTHF-binding, E149, was replaced by site-directed, mutagenesis demonstrate that MTHF acts in cryptochrome 3 as a functional, antenna for the photoreduction of FAD.
About this StructureAbout this Structure
2J4D is a Single protein structure of sequence from Arabidopsis thaliana with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Cryptochrome 3 from Arabidopsis thaliana: structural and functional analysis of its complex with a folate light antenna., Klar T, Pokorny R, Moldt J, Batschauer A, Essen LO, J Mol Biol. 2007 Feb 23;366(3):954-64. Epub 2006 Dec 2. PMID:17188299
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