Sandbox Reserved 402

This Sandbox is Reserved from September 14, 2021, through May 31, 2022, for use in the class Introduction to Biochemistry taught by User:John Means at the University of Rio Grande, Rio Grande, OH, USA. This reservation includes 5 reserved sandboxes (Sandbox Reserved 1590 through Sandbox Reserved 1594).

To get started: Click the edit this page tab at the top. Save the page after each step, then edit it again. Click the 3D button (when editing, above the wikitext box) to insert Jmol. show the Scene authoring tools, create a molecular scene, and save it. Copy the green link into the page. Add a description of your scene. Use the buttons above the wikitext box for bold, italics, links, headlines, etc. More help: Help:Editing. For an example of a student Proteopedia page, please see Photosystem II, Tetanospasmin, or Guanine riboswitch.

spinqualitylabelsall models Guanine Riboswitch Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Guanine RiboswitchGuanine Riboswitch

Riboswitches are highly conserved metabolite binding domains that are present in the 5'-UTR of certain mRNAs in bacteria. These structural elements bind specific metabolites in the aptamer (binding site) domain that results in allosteric rearrangement in the adjacent expression platform that interacts with RNA elements to regulate gene expression associated with biosythesis and transport.The operates by the binding of a metabolite to the aptamer domain which is highly conserved. Through allosteric effects the aptamer then changes the conformation of the expression platform which results in the premature termination of transcription, inhibition of translation initiation, or mRNA self-cleavage. Thus, riboswitches have two distinct conformations in which they operate: a metabolite bound and metabolite-free folds, involving the alternative base-pairing of the regulatory RNA region.

Riboswitch StructureRiboswitch Structure

In Bacillus subtilis, the 5'-UTR of xpt-pbuX mRNA binds guanine with high precision to down-regulate the expression of genes by forming transcription terminator structures.