Barstar

Crystal Structure of Barstar 2hxx

Barstar (BST) is an inhibitor synthesized and secreted by the bacterium Bacillus amyloliquefaciens. It binds to the ribonuclease barnase (BA) very tightly and prevents it from digestion of the cell’s RNA.

3D Structures of Barstarr3D Structures of Barstarr

2hxx, 2cx6 – BST
1a19 – BST (mutant)
1l1k, 1bta, 1btb – BST – NMR
1ab7 - BST (mutant) - NMR

BST + BABST + BA

3da7 – BST + BA (mutant)
2za4, 1brs, 1bgs – BST + BA
1x1u, 1x1w, 1x1x, 1x1y, 1b27, 1b2u – BST (mutant) + BA
1b3s, 1b2s - BST (mutant) + BA (mutant)

1ay7 – BST + guanyl-specific RNase SA – Streptomyces aureofaciens

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Michal Harel, Alexander Berchansky