1gkl
|
S954A MUTANT OF THE FERULOYL ESTERASE MODULE FROM CLOSTRIDIUM THERMOCELLUM COMPLEXED WITH FERULIC ACID
OverviewOverview
BACKGROUND: Degradation of the plant cell wall requires the synergistic, action of a consortium of predominantly modular enzymes. In Clostridiae, these biocatalysts are organized into a supramolecular assembly termed a, "cellulosome." This multienzyme complex possesses, in addition to its, well-described cellulolytic activity, an apparatus specific for xylan, degradation. Cinnamic acid esterases hydrolyze the ferulate groups, involved in the crosslinking of arabinoxylans to lignin and thus play a, key role in the degradation of the plant cell wall in addition to having, promising industrial and medical applications. RESULTS: We have cloned and, overexpressed the feruloyl esterase module from a 5 domain xylanase, Xyn10B from Clostridium thermocellum. The native structure at 1.6 A, resolution has been solved with selenomethionine multiple wavelength, anomalous dispersion and refined to a final R(free) of 17.8%. The, structure of a hydrolytically inactive mutant, S954A, in complex with the, reaction product ferulic acid has been refined at a resolution of 1.4 A, with an R(free) of 16.0%. CONCLUSIONS: The C. thermocellum Xyn10B ferulic, acid esterase displays the alpha/beta-hydrolase fold and possesses a, classical Ser-His-Asp catalytic triad. Ferulate esterases are, characterized by their specificity, and the active center reveals the, binding site for ferulic acid and related compounds. Ferulate binds in a, small surface depression that possesses specificity determinants for both, the methoxy and hydroxyl ring substituents of the substrate. There appears, to be a lack of specificity for the xylan backbone, which may reflect the, intrinsic chemical heterogeneity of the natural substrate.
About this StructureAbout this Structure
1GKL is a Single protein structure of sequence from Clostridium thermocellum with CD, FER, GOL and ACY as ligands. Active as Endo-1,4-beta-xylanase, with EC number 3.2.1.8 Structure known Active Site: FEA. Full crystallographic information is available from OCA.
ReferenceReference
The structure of the feruloyl esterase module of xylanase 10B from Clostridium thermocellum provides insights into substrate recognition., Prates JA, Tarbouriech N, Charnock SJ, Fontes CM, Ferreira LM, Davies GJ, Structure. 2001 Dec;9(12):1183-90. PMID:11738044
Page seeded by OCA on Mon Nov 5 16:14:26 2007