UDP-GALACTOPYRANOSE MUTASE FROM MYCOBACTERIUM TUBERCULOSIS

File:1v0j.gif


1v0j, resolution 2.25Å

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OverviewOverview

Uridine diphosphogalactofuranose (UDP-Galf) is the precursor of the, d-galactofuranose sugar found in bacterial and parasitic cell walls, including those of many pathogens. UDP-Galf is made from, UDP-galactopyranose by the enzyme UDP-galactopyranose mutase. The enzyme, requires the reduced FADH- co-factor for activity. The structure of the, Mycobacterium tuberculosis mutase with FAD has been determined to 2.25 A., The structures of Klebsiella pneumoniae mutase with FAD and with FADH-, bound have been determined to 2.2 A and 2.35 A resolution, respectively., This is the first report of the FADH(-)-containing structure. Two, flavin-dependent mechanisms for the enzyme have been proposed, one, which, involves a covalent adduct being formed at the flavin and the other based, on electron ... [(full description)]

About this StructureAbout this Structure

1V0J is a [Single protein] structure of sequence from [Mycobacterium tuberculosis] with FAD and BCN as [ligands]. Active as [UDP-galactopyranose mutase], with EC number [5.4.99.9]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

ReferenceReference

Crystal structures of Mycobacteria tuberculosis and Klebsiella pneumoniae UDP-galactopyranose mutase in the oxidised state and Klebsiella pneumoniae UDP-galactopyranose mutase in the (active) reduced state., Beis K, Srikannathasan V, Liu H, Fullerton SW, Bamford VA, Sanders DA, Whitfield C, McNeil MR, Naismith JH, J Mol Biol. 2005 May 13;348(4):971-82. PMID:15843027

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