1nhc
Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger
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OverviewOverview
Endopolygalacturonase I is a processive enzyme, while the 60% sequence identical endopolygalacturonase II is not. The 1.70 A resolution crystal structure of endopolygalacturonase I reveals a narrowed substrate binding cleft. In addition, Arg96, a residue in this cleft previously shown to be critical for processivity, interacts with the substrate mimics glycerol and sulfate in several well-defined conformations in the six molecules in the asymmetric unit. From this we conclude that both Arg96 and the narrowed substrate binding cleft contribute to retaining the substrate while it moves through the active site after a cleavage event has occurred.
About this StructureAbout this Structure
1NHC is a Single protein structure of sequence from Aspergillus niger with , , and as ligands. Active as Polygalacturonase, with EC number 3.2.1.15 Full crystallographic information is available from OCA.
ReferenceReference
Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger., van Pouderoyen G, Snijder HJ, Benen JA, Dijkstra BW, FEBS Lett. 2003 Nov 20;554(3):462-6. PMID:14623112
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