1gsv
CRYSTAL STRUCTURE OF THE P65 CRYSTAL FORM OF PHOTOACTIVE YELLOW PROTEIN G47S MUTANT
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OverviewOverview
Crystallographic and spectroscopic analyses of three hinge-bending mutants, of the photoactive yellow protein are described. Previous studies have, identified Gly(47) and Gly(51) as possible hinge points in the structure, of the protein, allowing backbone segments around the chromophore to, undergo large concerted motions. We have designed, crystallized, and, solved the structures of three mutants: G47S, G51S, and G47S/G51S. The, protein dynamics of these mutants are significantly affected. Transitions, in the photocycle, measured with laser induced transient absorption, spectroscopy, show rates up to 6-fold different from the wild type protein, and show an additive effect in the double mutant. Compared with the native, structure, no significant conformational differences were observed in ... [(full description)]
About this StructureAbout this Structure
1GSV is a [Single protein] structure of sequence from [Halorhodospira halophila] with HC4 as [ligand]. Structure known Active Site: HC4. Full crystallographic information is available from [OCA].
ReferenceReference
Engineering photocycle dynamics. Crystal structures and kinetics of three photoactive yellow protein hinge-bending mutants., van Aalten DM, Haker A, Hendriks J, Hellingwerf KJ, Joshua-Tor L, Crielaard W, J Biol Chem. 2002 Feb 22;277(8):6463-8. Epub 2001 Nov 19. PMID:11714713
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