6fc3

Crystal structure of the eIF4E-p20 complex from Saccharomyces cerevisiaeCrystal structure of the eIF4E-p20 complex from Saccharomyces cerevisiae

Structural highlights

6fc3 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[IF4E_YEAST] Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. [CAF20_YEAST] Acts as an inhibitor of cap-dependent translation. Competes with eIF4G1/TIF4631 and EAP1 for binding to eIF4E/TIF45 and interferes with the formation of the eIF4F complex, inhibiting translation and stabilizing mRNA. Binding affinity for eIF4E/TIF45 is 10-fold less than that of eIF4G1/TIF4631. Required for induction of pseudohyphal growth in response to nitrogen limitation, probably by regulating STE12 translation.^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7]

References

↑ Cosentino GP, Schmelzle T, Haghighat A, Helliwell SB, Hall MN, Sonenberg N. Eap1p, a novel eukaryotic translation initiation factor 4E-associated protein in Saccharomyces cerevisiae. Mol Cell Biol. 2000 Jul;20(13):4604-13. PMID:10848587
↑ Ramirez CV, Vilela C, Berthelot K, McCarthy JE. Modulation of eukaryotic mRNA stability via the cap-binding translation complex eIF4F. J Mol Biol. 2002 May 10;318(4):951-62. PMID:12054793 doi:http://dx.doi.org/10.1016/S0022-2836(02)00162-6
↑ von der Haar T, McCarthy JE. Intracellular translation initiation factor levels in Saccharomyces cerevisiae and their role in cap-complex function. Mol Microbiol. 2002 Oct;46(2):531-44. PMID:12406227
↑ Park YU, Hur H, Ka M, Kim J. Identification of translational regulation target genes during filamentous growth in Saccharomyces cerevisiae: regulatory role of Caf20 and Dhh1. Eukaryot Cell. 2006 Dec;5(12):2120-7. Epub 2006 Oct 13. PMID:17041186 doi:http://dx.doi.org/EC.00121-06
↑ Ibrahimo S, Holmes LE, Ashe MP. Regulation of translation initiation by the yeast eIF4E binding proteins is required for the pseudohyphal response. Yeast. 2006 Oct-Nov;23(14-15):1075-88. PMID:17083129 doi:http://dx.doi.org/10.1002/yea.1415
↑ Altmann M, Schmitz N, Berset C, Trachsel H. A novel inhibitor of cap-dependent translation initiation in yeast: p20 competes with eIF4G for binding to eIF4E. EMBO J. 1997 Mar 3;16(5):1114-21. doi: 10.1093/emboj/16.5.1114. PMID:9118949 doi:http://dx.doi.org/10.1093/emboj/16.5.1114
↑ Ptushkina M, von der Haar T, Vasilescu S, Frank R, Birkenhager R, McCarthy JE. Cooperative modulation by eIF4G of eIF4E-binding to the mRNA 5' cap in yeast involves a site partially shared by p20. EMBO J. 1998 Aug 17;17(16):4798-808. PMID:9707439 doi:http://dx.doi.org/10.1093/emboj/17.16.4798

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OCA

[1] Cosentino GP, Schmelzle T, Haghighat A, Helliwell SB, Hall MN, Sonenberg N. Eap1p, a novel eukaryotic translation initiation factor 4E-associated protein in Saccharomyces cerevisiae. Mol Cell Biol. 2000 Jul;20(13):4604-13. PMID:10848587

[2] Ramirez CV, Vilela C, Berthelot K, McCarthy JE. Modulation of eukaryotic mRNA stability via the cap-binding translation complex eIF4F. J Mol Biol. 2002 May 10;318(4):951-62. PMID:12054793 doi:http://dx.doi.org/10.1016/S0022-2836(02)00162-6

[3] von der Haar T, McCarthy JE. Intracellular translation initiation factor levels in Saccharomyces cerevisiae and their role in cap-complex function. Mol Microbiol. 2002 Oct;46(2):531-44. PMID:12406227

[4] Park YU, Hur H, Ka M, Kim J. Identification of translational regulation target genes during filamentous growth in Saccharomyces cerevisiae: regulatory role of Caf20 and Dhh1. Eukaryot Cell. 2006 Dec;5(12):2120-7. Epub 2006 Oct 13. PMID:17041186 doi:http://dx.doi.org/EC.00121-06

[5] Ibrahimo S, Holmes LE, Ashe MP. Regulation of translation initiation by the yeast eIF4E binding proteins is required for the pseudohyphal response. Yeast. 2006 Oct-Nov;23(14-15):1075-88. PMID:17083129 doi:http://dx.doi.org/10.1002/yea.1415

[6] Altmann M, Schmitz N, Berset C, Trachsel H. A novel inhibitor of cap-dependent translation initiation in yeast: p20 competes with eIF4G for binding to eIF4E. EMBO J. 1997 Mar 3;16(5):1114-21. doi: 10.1093/emboj/16.5.1114. PMID:9118949 doi:http://dx.doi.org/10.1093/emboj/16.5.1114

[7] Ptushkina M, von der Haar T, Vasilescu S, Frank R, Birkenhager R, McCarthy JE. Cooperative modulation by eIF4G of eIF4E-binding to the mRNA 5' cap in yeast involves a site partially shared by p20. EMBO J. 1998 Aug 17;17(16):4798-808. PMID:9707439 doi:http://dx.doi.org/10.1093/emboj/17.16.4798

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