3v8y

Structure of apo-glycogenin truncated at residue 270Structure of apo-glycogenin truncated at residue 270

Structural highlights 3v8y is a 1 chain structure with sequence from European rabbit. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: , Related: 1ll0, 1ll2, 1ll3, 1zct, 1zcu, 1zcv, 1zcy, 3v8z, 3v90, 3v91 Gene: GYG, GYG1 (European rabbit) Activity: Glycogenin glucosyltransferase, with EC number 2.4.1.186 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [GLYG_RABIT] Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase. Publication Abstract from PubMed The X-ray structure of rabbit glycogenin containing the T82M (T83M according to previous authors amino acid numbering [1]) mutation causing glycogenosis showed the loss of Thr82 hydrogen bond to Asp162, the residue involved in the activation step of the glucose transfer reaction mechanism. Autoglucosylation, maltoside transglucosylation and UDP-glucose hydrolyzing activities were abolished even though affinity and interactions with UDP-glucose and positioning of Tyr194 acceptor were conserved. Substitution of Thr82 for serine but not for valine restored the maximum extent of autoglucosylation as well as transglucosylation and UDP-glucose hydrolysis rate. Results provided evidence sustaining the essential role of the lost single hydrogen bond for UDP-glucose activation leading to glycogenin-bound glycogen primer synthesis. Structural and biochemical insight into glycogenin inactivation by the glycogenosis-causing T82M mutation.,Carrizo ME, Romero JM, Issoglio FM, Curtino JA FEBS Lett. 2012 Jan 3. PMID:22226635 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Glycogenin

spinqualitylabelsall models 3v8y, resolution 2.15Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image