Template:STRUCTURE 1ixg

File:1ixg.gif

PHOSPHATE-BINDING PROTEIN MUTANT WITH THR 141 REPLACED BY ASP (T141D), COMPLEXED WITH PHOSPATE


OverviewOverview

A very short hydrogen bond between an Asp and a phosphate is established in two high resolution structures (0.98 and 1.05 A). A mutant complex that changes the Asp to an Asn, which forms a normal hydrogen bond, has a similar free energy of binding to the wild type complex, suggesting that the contribution of the short hydrogen bond is not extraordinarily strong.

About this StructureAbout this Structure

1IXG is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

A low energy short hydrogen bond in very high resolution structures of protein receptor--phosphate complexes., Wang Z, Luecke H, Yao N, Quiocho FA, Nat Struct Biol. 1997 Jul;4(7):519-22. PMID:9228942 Page seeded by OCA on Fri May 2 20:32:40 2008

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