1a0i

ATP-DEPENDENT DNA LIGASE FROM BACTERIOPHAGE T7 COMPLEX WITH ATPATP-DEPENDENT DNA LIGASE FROM BACTERIOPHAGE T7 COMPLEX WITH ATP

Structural highlights 1a0i is a 1 chain structure. The July 2004 RCSB PDB Molecule of the Month feature on DNA Ligase by David S. Goodsell is 10.2210/rcsb_pdb/mom_2004_7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: Activity: DNA ligase (ATP), with EC number 6.5.1.1 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [DNLI_BPT7] DNA ligase, which is expressed in the early stage of lytic development, has been implicated in T7 DNA synthesis and genetic recombination. It may also play a role in T7 DNA repair. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The crystal structure of the ATP-dependent DNA ligase from bacteriophage T7 has been solved at 2.6 A resolution. The protein comprises two domains with a deep cleft running between them. The structure of a complex with ATP reveals that the nucleotide binding pocket is situated on the larger N-terminal domain, at the base of the cleft between the two domains of the enzyme. Comparison of the overall domain structure with that of DNA methyltransferases, coupled with other evidence, suggests that DNA also binds in this cleft. Since this structure is the first of the nucleotidyltransferase superfamily, which includes the eukaryotic mRNA capping enzymes, the relationship between the structure of DNA ligase and that of other nucleotidyltransferases is also discussed. Crystal structure of an ATP-dependent DNA ligase from bacteriophage T7.,Subramanya HS, Doherty AJ, Ashford SR, Wigley DB Cell. 1996 May 17;85(4):607-15. PMID:8653795 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also ATP-Dependent DNA Ligase (Bacteriophage T7) ATP-dependent DNA ligase from bacteriophage T7 DNA ligase

spinqualitylabelsall models 1a0i, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image