1e6e
ADRENODOXIN REDUCTASE/ADRENODOXIN COMPLEX OF MITOCHONDRIAL P450 SYSTEMS
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| , resolution 2.3Å | |||||||
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| Sites: | , , and | ||||||
| Ligands: | , , | ||||||
| Activity: | Ferredoxin--NADP(+) reductase, with EC number 1.18.1.2 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
OverviewOverview
The steroid hydroxylating system of adrenal cortex mitochondria consists of the membrane-attached NADPH-dependent adrenodoxin reductase (AR), the soluble one-electron transport protein adrenodoxin (Adx), and a membrane-integrated cytochrome P450 of the CYP11 family. In the 2.3-A resolution crystal structure of the Adx.AR complex, 580 A(2) of partly polar surface are buried. Main interaction sites are centered around Asp(79), Asp(76), Asp(72), and Asp(39) of Adx and around Arg(211), Arg(240), Arg(244), and Lys(27) of AR, respectively. In particular, the region around Asp(39) defines a new protein interaction site for Adx, similar to those found in plant and bacterial ferredoxins. Additional contacts involve the electron transfer region between the redox centers of AR and Adx and C-terminal residues of Adx. The Adx residues Asp(113) to Arg(115) adopt 3(10)-helical conformation and engage in loose intermolecular contacts within a deep cleft of AR. Complex formation is accompanied by a slight domain rearrangement in AR. The [2Fe-2S] cluster of Adx and the isoalloxazine rings of FAD of AR are 10 A apart suggesting a possible electron transfer route between these redox centers. The AR.Adx complex represents the first structure of a biologically relevant complex between a ferredoxin and its reductase.
About this StructureAbout this Structure
1E6E is a Protein complex structure of sequences from Bos taurus. The following page contains interesting information on the relation of 1E6E with [Cytochrome p450]. Full crystallographic information is available from OCA.
ReferenceReference
Adrenodoxin reductase-adrenodoxin complex structure suggests electron transfer path in steroid biosynthesis., Muller JJ, Lapko A, Bourenkov G, Ruckpaul K, Heinemann U, J Biol Chem. 2001 Jan 26;276(4):2786-9. Epub 2000 Oct 25. PMID:11053423 [[Category: [2fe-2s]ferredoxin]]
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