3pzd
Structure of the myosin X MyTH4-FERM/DCC complexStructure of the myosin X MyTH4-FERM/DCC complex
Structural highlights
Publication Abstract from PubMedMyosin X (MyoX), encoded by Myo10, is a representative member of the MyTH4-FERM domain-containing myosins, and this family of unconventional myosins shares common functions in promoting formation of filopodia/stereocilia structures in many cell types with unknown mechanisms. Here, we present the structure of the MyoX MyTH4-FERM tandem in complex with the cytoplasmic tail P3 domain of the netrin receptor DCC. The structure, together with biochemical studies, reveals that the MyoX MyTH4 and FERM domains interact with each other, forming a structural and functional supramodule. Instead of forming an extended beta-strand structure in other FERM binding targets, DCC_P3 forms a single alpha-helix and binds to the alphabeta-groove formed by beta5 and alpha1 of the MyoX FERM F3 lobe. Structure-based amino acid sequence analysis reveals that the key polar residues forming the inter-MyTH4/FERM interface are absolutely conserved in all MyTH4-FERM tandem-containing proteins, suggesting that the supramodular nature of the MyTH4-FERM tandem is likely a general property for all MyTH4-FERM proteins. Cargo recognition mechanism of myosin X revealed by the structure of its tail MyTH4-FERM tandem in complex with the DCC P3 domain.,Wei Z, Yan J, Lu Q, Pan L, Zhang M Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3572-7. Epub 2011 Feb 14. PMID:21321230[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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