2nn6
| |||||||
| , resolution 3.35Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | PMSCL1 (Homo sapiens), EXOSC4, RRP41, SKI6 (Homo sapiens), EXOSC8, OIP2, RRP43 (Homo sapiens), EXOSC5, CML28, RRP46 (Homo sapiens), EXOSC7, KIAA0116, RRP42 (Homo sapiens), EXOSC6 (Homo sapiens), EXOSC3, RRP40 (Homo sapiens), EXOSC2, RRP4 (Homo sapiens), EXOSC1, CSL4 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the human RNA exosome composed of Rrp41, Rrp45, Rrp46, Rrp43, Mtr3, Rrp42, Csl4, Rrp4, and Rrp40
OverviewOverview
The RNA exosome is a multisubunit 3' to 5' exoribonuclease complex that participates in degradation and processing of cellular RNA. To determine the activities and structure of the eukaryotic exosome, we report the reconstitution of 9-subunit exosomes from yeast and human and reconstitution of 10- and 11-subunit exosomes from yeast. Comparative biochemical analysis between purified subunits and reconstituted exosomes using AU-rich, polyadenylated (poly[A]), generic, and structured RNA substrates reveals processive phosphorolytic activities for human Rrp41/Rrp45 and the 9-subunit human exosome, processive hydrolytic activities for yeast Rrp44 and the yeast 10-subunit exosome, distributive hydrolytic activities for Rrp6, and processive and distributive hydrolytic activities for the yeast 11-subunit exosome. To elucidate the architecture of a eukaryotic exosome, its conserved surfaces, and the structural basis for RNA decay, we report the X-ray structure determination for the 286 kDa nine-subunit human exosome at 3.35 A.
About this StructureAbout this Structure
2NN6 is a Protein complex structure of sequences from Homo sapiens. The following page contains interesting information on the relation of 2NN6 with [Exosomes]. Full crystallographic information is available from OCA.
ReferenceReference
Reconstitution, activities, and structure of the eukaryotic RNA exosome., Liu Q, Greimann JC, Lima CD, Cell. 2006 Dec 15;127(6):1223-37. PMID:17174896
Page seeded by OCA on Thu Mar 20 17:48:28 2008