2i9t
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| , resolution 2.80Å | |||||||
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| Gene: | Rela, Nfkb3 (Mus musculus), Nfkb1 (Mus musculus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of NF-kB p65-p50 heterodimer bound to PRDII element of B-interferon promoter
OverviewOverview
Upon viral infection, NF-kappaB translocates to the nucleus and activates the IFN-beta gene by binding to the PRDII element. Strikingly, NF-kappaB loses its ability to activate the IFN-beta gene when the PRDII element is substituted by closely related sites. We report here the crystal structure of NF-kappaB p50/p65 heterodimer bound to the PRDII element from the IFN-beta promoter. The structure reveals an unexpected alteration in configuration, in which the p50 specificity domain moves by as much as approximately 9 A when compared to NF-kappaB heterodimer bound to the immunoglobulin kappaB site (Ig-kappaB) while maintaining the same base-specific contacts with the DNA. Taken together, the structure offers new insights into the allosteric effects of closely related DNA sites on the configuration of NF-kappaB and its transcriptional selectivity.
About this StructureAbout this Structure
2I9T is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Structure of NF-kappaB p50/p65 heterodimer bound to the PRDII DNA element from the interferon-beta promoter., Escalante CR, Shen L, Thanos D, Aggarwal AK, Structure. 2002 Mar;10(3):383-91. PMID:12005436
Page seeded by OCA on Thu Mar 20 17:26:56 2008