3an1

Crystal structure of rat D428A mutant, urate bound formCrystal structure of rat D428A mutant, urate bound form

Structural highlights

3an1 is a 2 chain structure with sequence from Rattus norvegicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , , , ,
Related:	3am9, 3amz
Resources:	FirstGlance, OCA, RCSB, PDBsum

Publication Abstract from PubMed

Two contradictory models have been proposed for the binding mode of the substrate xanthine to and its activation mechanism by xanthine oxidoreductase. In an effort to distinguish between the two models, we determined the crystal structures of the urate complexes of the demolybdo-form of the D428A mutant of rat xanthine oxidoreductase at 1.7 A and of the reduced bovine milk enzyme at 2.1 A, the latter representing a reaction intermediate. The results clearly indicate the catalytically relevant binding mode of the substrate xanthine.

Crystal Structures of Urate Bound Form of Xanthine Oxidoreductase: Substrate Orientation and Structure of the Key Reaction Intermediate.,Okamoto K, Kawaguchi Y, Eger BT, Pai EF, Nishino T J Am Chem Soc. 2010 Nov 15. PMID:21077683^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Okamoto K, Kawaguchi Y, Eger BT, Pai EF, Nishino T. Crystal Structures of Urate Bound Form of Xanthine Oxidoreductase: Substrate Orientation and Structure of the Key Reaction Intermediate. J Am Chem Soc. 2010 Nov 15. PMID:21077683 doi:10.1021/ja1077574

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OCA

[1] Okamoto K, Kawaguchi Y, Eger BT, Pai EF, Nishino T. Crystal Structures of Urate Bound Form of Xanthine Oxidoreductase: Substrate Orientation and Structure of the Key Reaction Intermediate. J Am Chem Soc. 2010 Nov 15. PMID:21077683 doi:10.1021/ja1077574

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