1qnt
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X-RAY STRUCTURE OF HUMAN O6ALKYLGUANINE-DNA ALKYLTRANSFERASE
OverviewOverview
The mutagenic and carcinogenic effects of simple alkylating agents are, mainly due to O(6)-alkylation of guanine in DNA. This lesion results in, transition mutations. In both prokaryotic and eukaryotic cells, repair is, effected by direct reversal of the damage by a suicide protein, O(6)-alkylguanine-DNA alkyltransferase. The alkyltransferase removes the, alkyl group to one of its own cysteine residues. However, this mechanism, for preserving genomic integrity limits the effectiveness of certain, alkylating anticancer agents. A high level of the alkyltransferase in many, tumour cells renders them resistant to such drugs. Here we report the, X-ray structure of the human alkyltransferase solved using the technique, of multiple wavelength anomalous dispersion. This structure explains the, ... [(full description)]
About this StructureAbout this Structure
1QNT is a [Single protein] structure of sequence from [Homo sapiens]. Active as [[protein-cysteine_S-methyltransferase Methylated-DNA--[protein]-cysteine S-methyltransferase]], with EC number [2.1.1.63]. Structure known Active Site: ACC. Full crystallographic information is available from [OCA].
ReferenceReference
Crystal structure of the human O(6)-alkylguanine-DNA alkyltransferase., Wibley JE, Pegg AE, Moody PC, Nucleic Acids Res. 2000 Jan 15;28(2):393-401. PMID:10606635 [[Category: Methylated-DNA--[protein]-cysteine S-methyltransferase]]
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