Maltose-binding protein

Maltose-binding protein (MBP) is part of the maltose/maltodextrin system in E. coli which is responsible of the catabolism of maltodextrin. MBP binds maltodextrin when the latter diffuses into the periplasm and passes it to proteins involved in active transport. MBP is widely used in molecular biology as it adds to the solubility of the proteins expressed in E. coli by being fused to them. The MBP-fusion protein can be purified by binding it to an amylose column followed by elution with maltose.

3D structures of maltose-binding protein3D structures of maltose-binding protein

1llx - MBP
3sey, 3sex, 3sew, 3sev, 3seu, 3set, 3ses, 3ser, 2v93, 1zkb, 1n3x, 1peb – MBP (mutant)
2klf, 2d21 – MBP – NMR
1ziu – MBP (mutant) + Ni
1zjl – MBP (mutant) + Zn
1zmg - MBP (mutant) + Cu

MBP complex with saccharide

1n3w, 1nl5 - MBP (mutant) + maltose
3hpi – MBP (mutant) + sucrose
2h25 - MBP + β-cyclodextrin
1fqa, 1fqb, 1fqc, 1fqd - MBP + maltotetraitol

MBP complex with protein

3pgf – MBP (mutant) + synthetic antigen binder
2r6g - MBP + maltose transporter complex
3puv, 3puw, 3pux – MBP + maltose transporter complex + ADP
3rlf - MBP + maltose transporter complex + Mg-AMPPNP
1svx – MBP + ankyrin repeat protein OFF7

MBP fused to other proteins

3hst – MBP/RNase H domain
3mq9 – MBP/bone marrow stromal antigen 2
3lbs - MBP/renin receptor + maltose
3lc8 - MBP/renin receptor
3csb – MBP/monobody YSX1
3csg - MBP/monobody YS1
2obg - MBP/monobody MBP-74
3f5f - MBP/Heparan sulfate 2-O-sulfotransferase
3g7v, 3g7w - MBP/islet amyloid polypeptide
2zxt, 3a3c - MBP/mitochondrial intermembrane space import and assembly protein 40
1ytv - MBP/vasopressin receptor
1r6z– MBP/argonaute 2
1hsj - MBP/accessary regulator protein R
3osq, 3osr – MBP/Green fluorescent protein