4ac5

Lipidic sponge phase crystal structure of the Bl. viridis reaction centre solved using serial femtosecond crystallographyLipidic sponge phase crystal structure of the Bl. viridis reaction centre solved using serial femtosecond crystallography

Structural highlights

4ac5 is a 4 chain structure with sequence from Blastochloris viridis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , , , ,
NonStd Res:
Related:	7prc, 1dxr, 2wjm, 1prc, 2x5v, 1vrn, 3prc, 6prc, 1r2c, 2wjn, 2x5u, 5prc, 2prc, 2jbl
Resources:	FirstGlance, OCA, RCSB, PDBsum

Publication Abstract from PubMed

X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.

Lipidic phase membrane protein serial femtosecond crystallography.,Johansson LC, Arnlund D, White TA, Katona G, Deponte DP, Weierstall U, Doak RB, Shoeman RL, Lomb L, Malmerberg E, Davidsson J, Nass K, Liang M, Andreasson J, Aquila A, Bajt S, Barthelmess M, Barty A, Bogan MJ, Bostedt C, Bozek JD, Caleman C, Coffee R, Coppola N, Ekeberg T, Epp SW, Erk B, Fleckenstein H, Foucar L, Graafsma H, Gumprecht L, Hajdu J, Hampton CY, Hartmann R, Hartmann A, Hauser G, Hirsemann H, Holl P, Hunter MS, Kassemeyer S, Kimmel N, Kirian RA, Maia FR, Marchesini S, Martin AV, Reich C, Rolles D, Rudek B, Rudenko A, Schlichting I, Schulz J, Seibert MM, Sierra RG, Soltau H, Starodub D, Stellato F, Stern S, Struder L, Timneanu N, Ullrich J, Wahlgren WY, Wang X, Weidenspointner G, Wunderer C, Fromme P, Chapman HN, Spence JC, Neutze R Nat Methods. 2012 Jan 29. doi: 10.1038/nmeth.1867. PMID:22286383^[1]

From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.

References

↑ Johansson LC, Arnlund D, White TA, Katona G, Deponte DP, Weierstall U, Doak RB, Shoeman RL, Lomb L, Malmerberg E, Davidsson J, Nass K, Liang M, Andreasson J, Aquila A, Bajt S, Barthelmess M, Barty A, Bogan MJ, Bostedt C, Bozek JD, Caleman C, Coffee R, Coppola N, Ekeberg T, Epp SW, Erk B, Fleckenstein H, Foucar L, Graafsma H, Gumprecht L, Hajdu J, Hampton CY, Hartmann R, Hartmann A, Hauser G, Hirsemann H, Holl P, Hunter MS, Kassemeyer S, Kimmel N, Kirian RA, Maia FR, Marchesini S, Martin AV, Reich C, Rolles D, Rudek B, Rudenko A, Schlichting I, Schulz J, Seibert MM, Sierra RG, Soltau H, Starodub D, Stellato F, Stern S, Struder L, Timneanu N, Ullrich J, Wahlgren WY, Wang X, Weidenspointner G, Wunderer C, Fromme P, Chapman HN, Spence JC, Neutze R. Lipidic phase membrane protein serial femtosecond crystallography. Nat Methods. 2012 Jan 29. doi: 10.1038/nmeth.1867. PMID:22286383 doi:10.1038/nmeth.1867

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OCA

[1] Johansson LC, Arnlund D, White TA, Katona G, Deponte DP, Weierstall U, Doak RB, Shoeman RL, Lomb L, Malmerberg E, Davidsson J, Nass K, Liang M, Andreasson J, Aquila A, Bajt S, Barthelmess M, Barty A, Bogan MJ, Bostedt C, Bozek JD, Caleman C, Coffee R, Coppola N, Ekeberg T, Epp SW, Erk B, Fleckenstein H, Foucar L, Graafsma H, Gumprecht L, Hajdu J, Hampton CY, Hartmann R, Hartmann A, Hauser G, Hirsemann H, Holl P, Hunter MS, Kassemeyer S, Kimmel N, Kirian RA, Maia FR, Marchesini S, Martin AV, Reich C, Rolles D, Rudek B, Rudenko A, Schlichting I, Schulz J, Seibert MM, Sierra RG, Soltau H, Starodub D, Stellato F, Stern S, Struder L, Timneanu N, Ullrich J, Wahlgren WY, Wang X, Weidenspointner G, Wunderer C, Fromme P, Chapman HN, Spence JC, Neutze R. Lipidic phase membrane protein serial femtosecond crystallography. Nat Methods. 2012 Jan 29. doi: 10.1038/nmeth.1867. PMID:22286383 doi:10.1038/nmeth.1867

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4ac5

Lipidic sponge phase crystal structure of the Bl. viridis reaction centre solved using serial femtosecond crystallographyLipidic sponge phase crystal structure of the Bl. viridis reaction centre solved using serial femtosecond crystallography

Structural highlights

Publication Abstract from PubMed

References

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4ac5

Lipidic sponge phase crystal structure of the Bl. viridis reaction centre solved using serial femtosecond crystallographyLipidic sponge phase crystal structure of the Bl. viridis reaction centre solved using serial femtosecond crystallography

Structural highlights

Publication Abstract from PubMed

References

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