2hhc
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Crystal structure of fucosyltransferase NodZ from Bradyrhizobium
OverviewOverview
The alpha-1,6-fucosyltransferase NodZ from Bradyrhizobium sp. WM9 (Lupinus), composed of 325 amino acids with a molecular weight of 37 kDa, has been cloned, expressed and purified. Protein crystals suitable for X-ray diffraction were obtained under optimized crystallization conditions using ammonium dihydrogen phosphate as a precipitant. The crystals are hexagonal and belong to space group P6(1)22 or P6(5)22, with unit-cell parameters a = 125.5, c = 95.6 A, and contain 56.8% solvent and a single protein molecule in the asymmetric unit. Native data were collected to 2.85 A using synchrotron radiation and cryogenic conditions. The native crystals were soaked in a mother-liquor solution containing 2.5 mM [Ta(6)Br(12)](2+) cluster for derivatization and SAD data were collected to 3.4 A at the tantalum L(III) absorption peak.
About this StructureAbout this Structure
2HHC is a Single protein structure of sequence from Bradyrhizobium sp. with and as ligands. Active as Glycoprotein 6-alpha-L-fucosyltransferase, with EC number 2.4.1.68 Full crystallographic information is available from OCA.
ReferenceReference
Cloning, purification, crystallization and preliminary crystallographic studies of Bradyrhizobium fucosyltransferase NodZ., Brzezinski K, Rogozinski B, Stepkowski T, Bujacz G, Jaskolski M, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):344-6. Epub 2004, Jan 23. PMID:14747720
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